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38 - ALKALINE NUTRIENT AGAR
To medium 5 add after sterilization 1 M Na-sesquicarbonate solution 1 ml to 10 ml to achieve pH 9.7 or 9% Na2Co3 solution 0.2 ml to 12 ml agar to achieve pH 9.5 - 10.0.
 
Na- sesquicarbonate solution
Distilled water100.0ml
Na2CO3 anhydrous5.3g
NaHCO34.2g
 
 

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