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Media
> Media 48
48 - ACETOBACTER MEDIUM
#Glucose solution
Dissolve 40 g glucose in 200 ml distilled water. Filter sterilize.
**Vitamin solution
Distilled water
100.0
ml
Biotin
0.2
mg
Folic acid
0.2
mg
p-Aminobenzoic acid
20.0
mg
Riboflavin
20.0
mg
Calsium pantothenate
40.0
mg
Thiamine hydrochloride
40.0
mg
Pyridoxine hydrochloride
40.0
mg
Nicotinic acid
40.0
mg
m-Inositol
200.0
mg
Filter-sterilize
*Trace element solution
Distilled water
100.0
ml
CuSO
4
x 5 H
2
O
7.8
mg
MnSO
4
x H
2
O
0.101
g
ZnSO
4
x 7 H
2
O
0.178
g
Na
2
MoO
4
x 2 H
2
O
23.4
mg
FeSO
4
x 7 H
2
O
0.366
g
CaCl
2
x 2 H
2
O
1.457
g
MgSO
4
x 7 H
2
O
0.25
g
Distilled water
800.0
ml
Agar
15.0
g
Glucose solution#
200.0
ml
Vitamin solution**
1.0
ml
Trace element solution*
1.0
ml
Yeast extract
5.0
g
KH
2
PO
4
1.0
g
(NH
4
)
2
SO
4
3.3
g
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