Online catalogue

64 - TRYPTONE YEAST EXTRACT MEDIUM
#Trace element solution
Distilled water5.0ml
NiCl2 x 6 H2O50.0ng
H3BO320.0ng
Na2MoO4 x 2 H2O50.0ng
CuCl2 x 2 H2O50.0mg
ZnCl20.2mg
CoCl2 x 6 H2O0.3mg
MnCl2 x 4 H2O0.5mg
FeCl2 x 4 H2O1.0mg
Titriplex I12.8mg
 
 
**Phosphate buffer
Distilled water1.0l
Na2HPO4 x 12 H2O43.0g
KH2PO45.44g
Autoclave phosphate buffer separately.
 
*Base medium
Distilled water1.0l
Trace element solution#5.0ml
Fe-citrate (0.01 M)5.0ml
MgCl2 x 6 H2O2.0g
CaSO4 x 2 H2O0.4g
NaOH-pellets0.2g
Titriplex I1.0g
 
 
Agar28.0g
Phosphate buffer**100.0ml
Distilled water800.0ml
Base medium*100.0ml
NaCl10.0g
Yeast extract2.5g
Tryptone2.5g
 
 

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