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68 - YEAST SYNTHETIC COMPLETE MEDIUM (SC)
*Synthetic stock solution
Distilled water300.0ml
Valine1.17g
Uracil0.22g
Tyrosine0.18g
Tryptophan0.82g
Threonine1.19g
Serine1.05g
Phenylalanine0.83g
Methionine1.49g
Lysine0.91g
Leucine2.62g
Isoleucine5.25g
Myo-inositol0.36g
Histidine0.58g
Aspartic acid2.66g
Arginine3.48g
Adenine0.135g
Storage in darkness at room temperature.
 
Agar20.0g
Synthetic stock solution*30.0ml
40% (w/v) glucose solution50.0ml
Milli-Q water920.0ml
Yeast Nitrogen Base w/o amino acids*6.7g
*Difco 0919. Glucose and synthetic stock solutions are filter sterilized and added after autoclaving.
 

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