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72 - STYRENE MINERAL SALTS MEDIUM
Agar, for solid medium20.0g
Milli-Q water1.0l
MnCl2 x 2 H2O1.0mg
CoCl2 x 6 H2O0.4mg
CuSO4 x 5 H2O0.2mg
Na2MoO4 x 2 H2O0.2mg
FeSO4 x 7 H2O5.0mg
CaCl2 x 2 H2O1.0mg
ZnSO4 x 7 H2O2.0mg
EDTA10.0mg
MgCl2 x 6 H2O0.1g
(NH4)2SO42.0g
NaH2PO4 x 2 H2O0.85g
K2HPO41.55g
Shake flask cultures: before inculation add in a fume cupboard 5 ml styrene into 50 ml sterile mineral salts solution and close tightly. Agar plates: incubation takes place in a dessicator. Add to the dessicator an open bottle containing 10 ml dibutyl phthalate and 200 ml styrene.
 

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