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> Media 74
74 - ACETAMIDE MEDIUM
*Trace element solution
Distilled water to
1.0
l
CoCl
2
x 6 H
2
O
3.7
g
ZnSO
4
x 7 H
2
O
1.4
g
MnSO
4
x H
2
O
1.6
g
FeSO
4
x 7 H
2
O
5.0
g
Distilled water
1.0
l
Agar (Noble)
20.0
g
Trace element solution*
1.0
ml
KH
2
PO
4
15.0
g
Glucose
20.0
g
Add after autoclaving (121°C/15 min)
1 M CSCl
2
12.5
ml
1 M Acetamide
10.0
ml
1 M CaCl
2
x 2 H
2
O
4.1
ml
1 M MgSO
4
x 7 H
2
O
2.4
ml
Autoclave solutions separately.
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