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| 74 - ACETAMIDE MEDIUM | ||
| *Trace element solution | ||
| Distilled water to | 1.0 | l |
| CoCl2 x 6 H2O | 3.7 | g |
| ZnSO4 x 7 H2O | 1.4 | g |
| MnSO4 x H2O | 1.6 | g |
| FeSO4 x 7 H2O | 5.0 | g |
| Distilled water | 1.0 | l |
| Agar (Noble) | 20.0 | g |
| Trace element solution* | 1.0 | ml |
| KH2PO4 | 15.0 | g |
| Glucose | 20.0 | g |
| Add after autoclaving (121°C/15 min) | ||
| 1 M CSCl2 | 12.5 | ml |
| 1 M Acetamide | 10.0 | ml |
| 1 M CaCl2 x 2 H2O | 4.1 | ml |
| 1 M MgSO4 x 7 H2O | 2.4 | ml |
| Autoclave solutions separately. | ||