Dissolve beef extract and peptone by heating in tap water, adjust pH to
7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at
121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water.
Liquid medium without agar should be combined when cooled to room
temperature. *Natural sea water is stored in the dark for at least three
weeks to "age". If natural sea water is not available use artificial sea
water.
