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91 - SEA WATER AGAR
Sea water*750.0ml
Tap water250.0ml
Agar20.0g
Peptone10.0g
Beef extract10.0g
Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
 
Artificial sea water
Distilled water1.00l
MgSO4 x 7 H2O3.50g
NaHCO30.11g
MgCl2 x 6 H2O4.80g
CaCl2 x 2 H2O1.60g
KCl0.77g
NaCl28.13g
 
 

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