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93 - BIFIDOBACTERIUM MEDIUM
Distilled water950ml
Resazurin (25 mg/100 ml)4ml
Salt solution (see below)40ml
Cysteine-HCl x H2O0.5g
NaCl5.0g
Tween 801.0ml
MnSO4 x H2O0.05g
MgSO4 x 7 H2O0.2g
K2HPO42.0g
Glucose10.0g
Bacto Soytone5.0g
Yeast extract5.0g
Meat extract5.0g
Casein peptone, tryptic digest10.0g
The cysteine is added after the medium has been boiled and cooled under CO 2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
 
Salt solution
Distilled water1.00l
NaCl2.00g
NaHCO310.00g
KH2PO41.00g
K2HPO41.00g
MgSO4 x 7 H2O0.50g
CaCl2 x 2 H2O0.25g
 
 

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